Application

Research CategorySignaling

Western blot: 0.5-2.0 µg/mL. MW = 180 kDa.

Flow cytometry: Dilute the antibody to 25 µg/mL and use 10-20 µL antibody for 1-2 x 10E5 cells in a total volume of 200-250 µL. Visualize with appropriate secondary antibodies.

ELISA: 1:10,000 - 1:100,000, using 50-100ng of control peptide/well.

Optimal working dilutions must be determined by end user.

Anti-VEGF Receptor-1 Antibody is an antibody against VEGF Receptor-1 for use in ELISA & WB.

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Human recombinant Flt-1 (extracellular domain). Actual epitope recognized by this clone is between 1-250 aa of human Flt-1.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified from Ascites. Liquid in PBS and 5% Trehalose.

Format: Purified

Specificity

Recognizes Human Flt-1 (fms-like tyrosine kinase). Flt-1 (human 1338 aa, rat 1336 aa, and mouse 1330aa), a putative receptor protein tyrosine kinase, is a receptor for VEGF and PLGF. Flt-1 has also been described as VEGFR1 and Flk-2. It is type 1 membrane protein and is expressed in normal lung, placenta, liver, kidney, heart, and brain but not in tumor cells (Shibuya et al., 1990; Matsushime et al., 1987; Yamane et al., 1994; Finnerty et al., 1993; Choi et al., 1994; Plouet et al., 1989; Simon et al.,

1998; Sait et al., 1995; deVries et al., 1992).

SPECIES REACTIVITIES:

Antibody crossreacts with mouse Flt-1. No significant sequence homology is seen with human VEGF-R2/KDR or other related kinases. Antibody crossreactivity in other species is not established.

Storage and Stability

Maintain frozen at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze and thaw.